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American Society of Microbiology ABMM Sample Questions:

1. A 30-year-old female who recently returned from a hiking trip in the northeastern United States presents to her physician with a two-week history of a slowly expanding, erythematous rash with central clearing on her thigh. She also reports intermittent fatigue, arthralgias, and mild headache. Serological testing reveals a positive IgM and IgG response to Borrelia burgdorferi. Which of the following mechanisms is MOST significant in the pathogenesis of the clinical manifestations observed in this patient?

A) Intracellular replication within macrophages leading to disseminated infection
B) Evasion of phagocytosis through a thick polysaccharide capsule
C) Production of potent exotoxins leading to direct tissue damage
D) Induction of a strong pro-inflammatory cytokine response by the host

2. The direct fluorescent antibody (DFA) test is commonly used for the rapid detection of which respiratory pathogen directly from nasopharyngeal specimens?

A) Legionella pneumophila (serogroup 1 in sputum)
B) Influenza virus
C) Bordetella pertussis
D) Mycoplasma pneumoniae

3. A patient with a history of travel to South America presents with fever, lymphadenopathy, and hepatosplenomegaly. Peripheral blood smear reveals the presence of trypomastigotes. The MOST likely diagnosis is:

A) Leishmaniasis
B) African trypanosomiasis (sleeping sickness)
C) Malaria
D) Chagas disease (American trypanosomiasis)

4. A microbiology laboratory receives a sample of cerebrospinal fluid from a patient with suspected bacterial meningitis. The Gram stain shows Gram-positive diplococci. Culture on blood agar shows alpha-hemolytic colonies that are bile soluble and optochin-sensitive. The MOST likely organism is:

A) Streptococcus agalactiae
B) Streptococcus pneumoniae
C) Enterococcus faecalis
D) Listeria monocytogenes

5. A centrifuge rotor containing capped tubes of sputum concentrate for AFB culture breaks during operation inside a Class II Biological Safety Cabinet (BSC). What is the most appropriate immediate action for the technologist?

A) Open the centrifuge within the BSC, discard broken glass in a regular sharps container, and clean the spill with 10% bleach.
B) Keep the BSC running, notify the supervisor, evacuate the immediate area, and post warning signs.
Allow aerosols to settle (e.g., 30 min) before initiating decontamination with a tuberculocidal disinfectant.
C) Carefully remove any intact tubes, wipe them with 70% alcohol, and place them in a new rotor to continue processing.
D) Immediately turn off the BSC, open the sash fully, and spray disinfectant widely.

Solutions: